Music recognition app-maker Shazam, mobile security firm Revector and hi-fi maker Linn have all been honoured in the annual awards.
Shazam, an app which identifies songs when users scan for them, was recognised after becoming one of the most popular smartphone services. It was awarded in the Innovation category having recently passed 200 million users worldwide.
It has also recently teamed up with ITV to allow its users to detect what is playing in adverts and interact with the commercials.
Revector, which detects fraud in mobile phone billing, received the Queen's award in both the Innovation and International Trade categories.
It claims to be "at the forefront of anti-fraud technology".
Revector's CEO Andy Gent said he was "delighted to have been recognised in the Queen’s Diamond Jubilee year".
Hi-fi manufacturer Linn, which received a Royal Warrant in 2002 for supplying equipment to the Royal Household was given an innovation award for its upmarket range of music streaming devices.
Past winners of the award include Arm holdings, which make the processors in Apple's iPhone and iPad. The awards are seen as the most prestigious accolade a business can be given.